Description
Semaphorin 3C, also known as SemaE, is a member of the semaphorin family 3 that are grouped into eight major classes based on structure and phylogenetic tree analyses. It is expressed in all somitic motor neurons, in lung buds, and in cardiac neural crest cells during development. ?The functions of Semaphorin 3C are mediated primarily through binding to the Plexin-D1 and Neuropilin 1 or Neuropilin 2 coreceptor complex. Semaphorins are usually cues to deflect axons from inappropriate regions, especially important in the neural system development. Also, Semaphorin 3C activates integrins in certain cells, so in addition to its repulsive activities, it sometimes acts as a chemoattractant. Class 3 have an important function after traumatic central nervous system injuries, such as spinal cord injury. It regulate neuronal and non-neuronal cells associated with the traumatic injury due to their presence in the scar tissue. Recombinant human Semaphorin 3C, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques